- Human soluble Cluster of differentiation 100(sCD100)ELISA Kit
- CUSABIO Technology LLC
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- CSB-E15792h
- 96T
- 125 pg/mL-8000 pg/mL
- 450 nm
- Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
- serum, plasma, tissue homogenates
- 31.25 pg/mL
- 50-100ul
- sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
- 1-5h
- ELISA
- ELISA Kit
- Homo sapiens (Human)
- semaphorin 4D
- A8, BB18, C9orf164, CD100, COLL4, GR3, M-sema-G, SEMAJ, coll-4
- Neuroscience
Specifications/Features
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SEMA4D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEMA4D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA4D is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA4D bound in the initial step. The color development is stopped and the intensity of the color is measured.